INVITRO ANTI-OXIDANT CAPACITY OF YOYO BITTERS AND ITS NUTRITIONAL EFFECTS ON MALE ALBINO WISTAR RATS
ABSTRACT
In order to investigate invitro antioxidant capacity of yoyo bitters and its nutritional effect on the weight of male albino wistar rats. Sixteen(16) rats weighing (163±2.00g) were randomly picked into two equal group; (Group A and B). The rats were acclimatized for 7days, after which 1ml 0f yoyo bitters were administered once daily to group A respectively for the period of 28days while group B were received distilled water which serve as control. The feed was weighed throughout the period of the experiment. Each group were given 300g of mash grower feed daily and animals were also weighed weekly. At the end of 4week, a significant increase was observed in the weight of the test rat (208.08±9.37g) compared to control (192.69±10.20g). The relative weight of the following organ: Liver, Kidney, Heart, Lungs, Spleen, Testes and Pancreas were observed and its was statistically significant (P<0.05). The total antioxidant capacity was compare using T test, the value of the test and control are statistically significant (P<0.05), Hydroxyl free radical n al activity (P<0.05) and DPPH scavenging activity (P<0.05).
CHAPTER ONE
INTRODUCTION
Background of the study
Oxidative stress occurs when there is an imbalance between free radicals, reactive oxygen species (ROS), and the antioxidant defense system. This imbalance can lead to various pathological conditions, including inflammation, cardiovascular disease, diabetes, and cancer. The use of natural antioxidants from plants has gained attention in recent years, as they have been shown to have fewer side effects and can effectively neutralize free radicals. Yoyo Bitters is a herbal preparation commonly used in African traditional medicine to treat various ailments, including malaria, fever, and digestive disorders. It is formulated from several plant extracts, including Mahogany bark, Cinnamon, and Clove.
In vitro studies have shown that Yoyo Bitters possesses significant antioxidant activity, making it a promising natural remedy for oxidative stress-related disorders. However, limited research has been conducted to investigate the nutritional effects of Yoyo Bitters in vivo. Therefore, this study aimed to evaluate the in vitro anti-oxidant capacity of Yoyo Bitters and assess its potential nutritional effects on male albino Wistar rats
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